WebApr 7, 2024 · C Western blots show that DDX21 proteins are highly expressed in CRC tissues than adjacent normal tissues in ten CRC patients. β-actin is used as a loading control. Two biological replicates are... WebProduct Description. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays. Dojindo’s highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue ...
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WebThe cell number was then detected using CCK8 (Bimake, B34304) reagent by measuring the absorbance at 450 nm with a microplate reader (TECAN, Switzerland). For the colony formation assay, 1×103cells per well were seeded on 6-well plates in triplicate and maintained in medium containing 10% FBS. WebThe Cell Counting Kit-8 (CCK8) is a one-bottle kit that requires no premixing. This non-radioactive, sensitive colorimetric assay allows accurate live cell counting in a cell proliferation or cytotoxicity assay applications. CCK8 based assay has excellent correlation with results of traditional radioactive [ 3 H]-thymidine incorporation assay. marinetrafficlineshippingmvhebrideanisles
Cell Counting Kit - 8 for quantitation of viable cell number in ...
WebElabscience® Cell Counting Kit 8 (WST-8 / CCK8) is a rapid and highly sensitive kit based on WST-8, which is widely used in cell proliferation and cytotoxicity detection. Detection principle . WST-8 is a compound similar to MTT, which can be reduced to orange formazan by some dehydrogenase WebCell Counting Kit (CCK8, B34304) was purchased from Bimake (USA). Annexin V-FITC Apoptosis Detection Kit (C1062M) was purchased from Beyotime (Shang Hai, China). … WebJul 7, 2024 · Cell Counting Kit-8 (CCK8) (Selleck, B34304) is used in cell proliferation and metabolism. For the same cell types, the more cells proliferate, the stronger the cell viability and the higher OD value. Similarly, the higher cellular metabolic rate, the higher OD value will be obtained. Neonatal rat cardiomyocytes (NRCMs) were seeded in 96-well ... marine traffic lake ontario