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Dna ligands for aflatoxin and zearalenone

WebA sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p -aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. WebNov 1, 2024 · Anti-idiotypic nanobody-phage display-mediated real-time immuno-PCR for sensitive, simultaneous and quantitative detection of total aflatoxins and zearalenone in grains Anti-idiotypic nanobody-phage display-mediated real-time immuno-PCR for sensitive, simultaneous and quantitative detection of total aflatoxins and zearalenone in grains …

Linda Chryseis Le Inventions, Patents and Patent Applications

Webzearalenone aflatoxin dna ligands ligands dna Prior art date 2009-08-21 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Withdrawn Application number EP10809412.9A Other languages German (de) French (fr) WebJun 15, 2014 · Ochratoxin A (OTA), zearalenone (ZEN), α-zearalenol, and aflatoxin M1 (AFM 1) were purchased from Sigma-Aldrich (USA). Aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2), and fumonisin (FB1) were purchased from Pribolab Co. Ltd (Singapore). Streptavidin was obtained from Sangon Biotechnology Co. Ltd. (Shanghai, … prince\u0027s-feather 7n https://ticoniq.com

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WebDec 15, 2014 · AFB1 and related mycotoxins (AFB2, AFG1, AFG2, ochratoxin A, zearalenone, citrinin, T-2 toxin, deoxynivalenol, and patulin), bovine serum albumin (BSA), and sodium chloride were purchased from Sigma (St. Louis, MO, USA). 96-Microwell plates (flat bottom) were purchased from Nunc (Roskilde, Denmark). WebSep 15, 2024 · Aptamers are single strand oligonucleotides selected in vitro by Systematic Evolution of Ligands by Exponential ... Simultaneous detection of aflatoxin B1, ochratoxin A, zearalenone and deoxynivalenol in corn and wheat using surface plasmon resonance ... G.A. Penner, DNA ligands for aflatoxin and zearalenone, US patent … WebPreviously, we designed five new aptamers to aflatoxin B 1 (AFB1) based on a known aptamer sequence (Patent: PCT/CA2010/001 292, Apt1) through a genetic algorithm-based in silico maturation strategy and experimentally measured their affinity to the target toxin. Here, integrated molecular dynamic simulation (MDs) studies with molecular ... prince\\u0027s-feather 7n

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Dna ligands for aflatoxin and zearalenone

An In-Silico Pipeline for Rapid Screening of DNA Aptamers against ...

WebApr 15, 2024 · 1. Introduction. Mycotoxins are secondary metabolites of fungi. At present, about 400 mycotoxins have been identified and a dozen have been confirmed harmful to humans and animals, such as aflatoxin, ochratoxin, fumonisin, zearalenone, patulin and so on [1].Among them, aflatoxin is considered as one of the most significant mycotoxins … WebAug 20, 2010 · The present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural …

Dna ligands for aflatoxin and zearalenone

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WebJun 8, 2013 · Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin produced by Fusarium graminearum on maize and barley. Because most current methods of ZEN detection rely on the use of low-stability antibodies or expensive equipment, we sought to develop a rapid, low-cost determination method using aptamers instead of antibodies as … WebThe invention further relates to methods for identifying DNA ligands capable of binding to aflatoxin and zearalenone. The invention further relates to new DNA sequences.[origin: WO2011020248A1] The present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone.

WebAug 20, 2010 · DNA LIGANDS FOR AFLATOXIN AND ZEARALENONE. Abstract: The present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to … WebSep 1, 2024 · Aflatoxin B 1 and zearalenone were simultaneously determined with high selectivity. Abstract Mycotoxins contamination in agricultural products poses a serious threat to human and animal health, so rapid and sensitive nanosensors for simultaneous determination of multiple mycotoxins in food samples are highly desirable for food safety …

WebA method for the identification of DNA ligands that bind to Zea characterized in that the method comprises: (a) contacting a random library of single stranded DNA sequences to immobilized Zea under... WebFeb 19, 2024 · National Center for Biotechnology Information

WebDNA LIGANDS FOR AFLATOXIN AND ZEARALENONE United States Application US20120245494 Note: If you have problems viewing the PDF, please make sure you have the latest version of Adobe Acrobat . Back to full text

WebThe present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to methods for removing or reducing the level of aflatoxin and zearalenone … prince\\u0027s-feather 7oWebThe present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to methods for removing or reducing the level of aflatoxin and zearalenone … prince\u0027s-feather 7pWebAug 20, 2010 · The present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to methods for removing or reducing the level of aflatoxin and … prince\\u0027s-feather 7mWeb核酸适配体既可以是RNA也可以是DNA，广泛应用在食品安全检测中作为危害因子的特异性分子识别元件，在物质分离富集过程中作为能够与靶目标发生特异性结合的配基以及在医学上用于临床诊断和治疗。 ... 指数富集配基系统进化技术（Systematic Evolution Of Ligands by ... prince\\u0027s-feather 7pWebDec 1, 2016 · Aflatoxins are naturally occurring mycotoxins that contaminate food and agro commodities, leading to acute and chronic health conditions in human and animals. In the present work, an attempt was made to generate high-affinity single stranded DNA aptamers that specifically bind to Aflatoxin B1 (AFB1) by a modified Systemic Evolution of … prince\u0027s-feather 7rWebMar 8, 1999 · Aflatoxin B1 (AFB1), a category I known human carcinogen and the most potent genotoxic agent, is mutagenic in many model systems and produces chromosomal aberrations, micronuclei, sister chromatid exchange, unscheduled DNA synthesis, and chromosomal strand breaks, as well as forms adducts in rodent and human cells. prince\\u0027s-feather 7uWebDNALGANDS FOR AFLATOXIN AND ZEARALENONE FIELD OF THE INVENTION 0001. The invention relates to DNA ligands, and more particularly to DNA ligands capable of binding to aflatoxin and zearalenone. The present invention further relates to methods and use of DNA ligands capable of binding to afla toxin and Zearalenone. BACKGROUND … prince\u0027s-feather 7u