WebUsing the EGF receptor as a model, we show that EGF stimulation of receptor-mediated endocytosis and fluid-phase endocytosis is Rab5-dependent and is coupled to Rab5 … WebMay 21, 2014 · After adherence of hepatocytes for 3 h, the medium was replaced by ice-cold medium containing 10 ng ml −1 fluorescein-conjugated EGF (Life Technologies) and incubated for 30 min to allow ...
Epidermal growth factor (EGF) triggers nuclear calcium signaling ...
WebJul 19, 2015 · We also perform a series of experiments where, following the washes, fresh medium containing different concentrations of EGF (Life Technologies) is added to the wells. The concentrations of EGF we use are: 25, 50, 75, 100 and 125 ng/mL. We will refer to these assays as EGF-25, EGF-50, EGF-75, EGF-100 and EGF-125, respectively. WebNov 1, 2024 · stimulated with 50 ng/mL EGF (Life Technologies, #PHG0315) during the last 15 min of compound treatment. After washed. with cold PBS for 3 times, cells were lysed in SDS lysis buffer and. home goods child recliner
Human EGF Recombinant Protein (PHG0311)
WebOct 30, 2024 · Abstract. Tyrosine kinase receptors such as the epidermal growth factor receptor (EGFR) transduce information from the microenvironment into the cell and … WebOct 7, 2016 · This effect was EGF-dependent and attenuated with the EGF receptor inhibitor erlotinib. Hypoxia did not affect the capacity of neurospheres to generate neuron- or glia-like precursors. ... (1:200; both Life Technologies) were used in the control experiments (Additional file 2: Figure S2). Human BMSCs and NIH/3T3 mouse fibroblast line were … WebOct 1, 2000 · All cell culture reagents were obtained from Life Technologies. The growth medium consisted of minimum essential medium (α-MEM), ... Mouse EGF (Life Technologies) was iodinated with 125 I (NEN Life Science Products) using IODO-BEADS (Pierce Chemical Co.), according to the manufacturer's protocol. The specific activities of … home goods cincinnati locations