Webexcitation to emission, is measured in only billionths of a second, the phenomenon is a stunning manifestation of the interaction between light and matter that forms the basis for … Web195 rows · It is strongly urged that the data for a particular fluorochrome be confirmed by consulting the dye manufacturer to secure full specification of curves for excitation and …
Fluorescence - Flow Cytometry Guide Bio-Rad
WebExcitation (empty curves) and emission spectra (filled curves) for FITC excited with 488 nm blue laser (Aa) and mCherry excited with 561 nm yellow-green laser (Ab). FITC and mCherry... WebFITC exhibits an excitation maximum at λ = 495 nm and emission maximum at approximately λ = 519 nm. The color of the compound is yellow while the emitted light is green. There are two different isomers of FITC: Isomer I, also referred to as fluorescein 5-isothiocyanate or 5-FITC Isomer II, also referred to as fluorescein 6-isothiocyanate or 6 … starling contact number
Fluorescence Filter Set for FITC Fluorescein Edmund Optics
WebFluorophore Excitation filter Emission filter Mirror module Fluo-4 2100-5020 FITC 485/14 filter 2100-5120 FITC 535/25 filter 2100-4030 FITC D505 single mirror Fura-2 2100-5390 Fura2, BFP 380/10 filter 2100-5750 Photometric Fura-2 340/14 filter 2100-5320 Fura2 510/10 filter 2100-4170 LANCE/DELFIA D400 SINGLE MIRROR (e)GFP: (enhanced) … WebMar 17, 2024 · The excitation and emission wavelengths of FITC are 494nm and 518 nm, respectively, which gives it a green color. Originally, FITC was reported to have two isomers – fluorescein 5-isothiocyanate and fluorescein 6-isothiocyanate. A product of both isomers is also known to exist (fluorescein 5 (6)-isothiocyanate). WebMar 16, 2024 · That will lead to the most sensitivity and lowest detection limits in the analysis. For the spectra of anthracene drawn in Figure 3.4. 6, that would correspond to an excitation wavelength of 360 nm and emission wavelength of 402 nm. The one exception is if the S 0 -S 1 transition is the maximum on both spectra, which would mean having the ... starling construction inc