Web16 de dez. de 2024 · The linear DNA was cyclized into ΔKlcA HS pHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) … Web2 de mai. de 2024 · Carbapenemase-producing Klebsiella pneumoniae (KPC) has emerged and spread throughout the world. A retrospective analysis was performed on carbapenem-resistant K. pneumoniae isolated at our teaching hospital during the period 2009–2010, when the initial outbreak occurred. To determine the mechanism(s) that underlies the …
Gene editing of Duchenne muscular dystrophy using
Web翌圣生物以工具酶为核心,研发生产销售pcr,qpcr,高保真酶,热启动酶,一步法快速克隆试剂盒,一步法快速支原体检测试剂盒等分子、蛋白、细胞、免疫等3000多种试剂及试剂盒,现有科 … Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID fragment was amplified by PCR from a commercial gene (USA126049; Addgene, Watertown, MA, USA). The sequences of the primers were listed in Table S1. rita ora fifty shades freed song
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Web6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in the transformed E. coli by recombination according to HieffClone TM MultiOneStep Cloning kit (YEASEN, Shanghai, China). Web本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA片段的原理进一步研发而成,与传统的T4连接酶相比,具有以下优势:1)快速,仅需1-5分钟即可完成连接反应 … Web本试剂盒是利用拓扑异构酶 (Topoisomerase)高效快速连接DNA片段的原理进一步研发而成,与传统的T4连接酶相比,具有以下优势:1)快速,仅需 1-5 min即可完成连接反应。. … smiley face pot scrubbers