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Klf3 chip抗体

WebApr 14, 2024 · ChIP是利用抗原和抗体的特异性结合以及细菌蛋白质的“protein A”特异性地结合到免疫球蛋白的Fc片段的现象合用开发出来的方法(免疫磁珠结合proteinA,proteinA结合抗体Fc段,抗体结合抗原即发生特异标记的组蛋白,这里要注意组蛋白是和DNA结合的,这 … Web【課題】デュシェンヌ型筋ジストロフィーの処置のための材料および方法の提供。 【解決手段】本出願は、デュシェンヌ型筋ジストロフィー(DMD)を伴う患者を、ex vivoおよびin vivoの両方で処置するための材料および方法を提示する。

Comprehensive Investigation of the Molecular Mechanism of …

WebJul 24, 2024 · KLF3 binds to active enhancers proximal to differentiation genes that are dependent upon KLF3 for expression. KLF3's genomic binding sites also highly overlaps with CBP, a histone acetyltransferase necessary for activating enhancers. Depletion of KLF3 causes reduced CBP localization at enhancers proximal to differentiation gene clusters, … WebAntibodies that detect KLF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. These … nbc the office wallpaper pam https://ticoniq.com

KLF3抗体 Abcam中文官网

WebMay 16, 2014 · Conclusions. These findings reveal that the occupancy profile of KLF3 at the α-globin locus differs in erythroid and non-erythroid cells. In erythroid cells, KLF3 primarily binds to the promoters of the adult α-globin genes, but appears dispensable for normal transcriptional regulation. In non-erythroid cells, KLF3 distinctly binds to the HS-12 and HS … Web抗体的选择要注意三点:一是应该选择能够做ChIP实验的抗体。尽量选择ChIP级别商业化的抗体。如没有,一般可以重组到带有标签的载体上,再转染相应的宿主(目前市面上商业化的chip抗体只有300多种,因此正好找到对应抗体较困难,可以选择标签抗体)。 Web染色质免疫沉淀法 (chip) 是一种基于抗体的技术,可用来选择性地使特异性 dna 结合蛋白及其 dna 靶标富集。chip 可用来研究某种特殊的蛋白-dna 相互作用、多种蛋白-dna 相互作 … nbc the open

CD4 Mouse Monoclonal Antibody (CD4小鼠单抗)(AG1393)

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Klf3 chip抗体

KLF4 Antibody (NBP1-83940): Novus Biologicals

WebMar 21, 2024 · KLF3 (KLF Transcription Factor 3) is a Protein Coding gene. Diseases associated with KLF3 include Cutaneous Adenocystic Carcinoma. Gene Ontology (GO) … WebSep 19, 2024 · m6A抗体应用范围: 1、Western blot(1-4 g/ml); 2、Dot Blot. 3、ChIP, 4、RNA Binding Protein Immunoprecipitation (RIP). 《抗体结果展示》 泳道1:m6A偶联的BSA蛋白;泳道2:腺嘌呤偶联的BSA蛋白. 相关产品推荐: RNA甲基化(m6A)定量分析试剂盒 P-9005. DNA甲基化(m6A)定量分析试剂 ...

Klf3 chip抗体

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WebKrüppel-like Factor 3 (KLF3) is a transcriptional regulator that we have shown to be involved in the regulation of adipogenesis in vitro. Here we report that KLF3 null mice are lean and protected ... WebNational Center for Biotechnology Information

WebNov 5, 2024 · KLF3 induced cell cycle arrest and macrophage differentiation. We will report on KLF3-induced gene expression changes (repression and activation), and ChIP-seq for … WebKLF3, originally termed Basic Krüppel-like Factor (BKLF), was the third member of the Krüppel-like factor family of zinc finger transcription factors to be discovered. [5] …

WebThe left column represents KLF3 ChIP-seq reqad density and the center column represents KLF1 ChIP-seq read density. Regions were sorted according to hierarchical clustering using the nearest neighbour chain algorithm (95, 96). Clustering was based on both ChIP-seq data sets using values quantified in an area ranging from –50 bp to +50 bp from ... WebDec 3, 2015 · The band A, but not band B, disappeared or become weaken in the presence of anti-KLF3 antibody, indicates KLF3 interacts with SHP-1 P2 promoter. To test the ability of KLF3 binding to SHP-1 promoter 2 (P2) in vivo, we performed chromatin immunoprecipitation (ChIP) analysis using antibodies for KLF3 and compared it with the …

WebKLF3 Antibody (B-12) is a high quality monoclonal KLF3 antibody (also designated BKLF antibody) suitable for the detection of the KLF3 protein of mouse, rat and human origin. …

WebAnti-KLF3 antibody produced in rabbit IgG fraction of antiserum; Synonyms: 抗クルッペル様因子3(塩基性)抗体; find Sigma-Aldrich-AV32186 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich nbc the reckoningWeb免疫沉淀和质谱联用 (IP-MS),可用来确认抗体与预期靶标的特异性相互作用。. 在现有的抗体验证*方法中,这种方法的特别之处在于可以主动识别和定量纯化自样本的实际抗体靶标。. 与其他评估抗体特异性的技术(如,通过对基因敲除小鼠来源样本进行免疫印迹 ... marriage directoryWebDec 3, 2015 · In quiescent cell, KLF3 is a highly expressed transcriptional factor, but once T lymphocyte is activated, KLF3 expression is reduced to an undetectable level. The Src … nbc the place to be 1991WebCD4 Mouse Monoclonal Antibody -20ºC保存,需避免反复冻融。. Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。. 如果本抗体用于Western blot (WB)、免疫荧光 (IF ... marriage disciples catholicWebInterestingly, KLF3 protein is stabilized in a small subset of mESCs. Transcriptome analysis indicates that KLF3-positive mESCs upregulate genes that are initially activated in 8-cell … nbc there be thereWeb1. 实验原理. 免疫沉淀(Immunoprecipitation, IP) 是利用抗体特异性反应纯化富集目的蛋白的一种方法。. 抗体与细胞裂解液或表达上清中相应的蛋白结合后,再用ProteinA/G抗 … marriage dissolutions spokane countyWebIP, ChIP, WB, ICC/IF reported in scientific literature (PMID: 25228645). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF: fixation/permeabilization: PFA/Triton X-100. KLF4 antibody validated for WB from a verified customer review. Use in KD reported in scientific publication (PMID: 32427586). nbc there\u0027s good news tonight