Pcr2.1 topo vector
SpletComments for pCR®2.1-TOPO® 3931 nucleotides LacZα fragment: bases 1-547 M13 reverse priming site: bases 205-221 Multiple cloning site: bases 234-357 T7 … SpletThe pCR™ 2.1-TOPO™ TA vector is linearized with 3'-thymidine (T) overhangs for direct ligation of Taq polymerase–amplified PCR products (TA cloning™) and is 'activated' with …
Pcr2.1 topo vector
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Splet27. okt. 2016 · 1. Create Your PCR Product: Design standard primers (no need to add unique restriction sites on the ends) and amplify your sequence of interest with Taq polymerase using your favorite PCR protocol. 2. Set … SpletThe pCR 2.1-TOPO TA vector is linearized with 3'-thymidine (T) overhangs for direct ligation of Taq polymerase–amplified PCR products (TA cloning) and is “activated” with …
SpletFree Trial. TOPO Cloning Vectors. Commonly used TOPO sequences including blunt, directional, and TOPO TA cloning vectors. Download Plasmid Set. Try SnapGene for Free. … SpletBasic Vector Information; Vector Name: pCR2.1-TOPO: Antibiotic Resistance: Ampicillin Length: 3931 bp: Type: I.M.A.G.E. Consortium Plasmids Source: Invitrogen (Life …
SpletThermo Fisher pcr2 1 topo t a cloning shuttle vector Pcr2 1 Topo T A Cloning Shuttle Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … SpletDescription. Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants. 3'-T overhangs for direct ligation of Taq-amplified PCR products ; Choice of T7 (pCR2.1-TOPO™) or T7 and SP6 (pCRII-TOPO™) promoters for in vitro RNA transcription and sequencing.Each vector also contains M13 forward and reverse primer …
SpletTOPO® TA Cloning® Kit . Five-minute cloning of Taq polymerase-amplified PCR products . Catalog numbers (pCR ™ 2.1-TOPO ® vector) K4500-01, K4500-40, K4500-J10, K4510 …
Splet15. nov. 2007 · The TOPO TA vectors have an topisomerase attached so all you do is incubate your insert and the vector with a salt solution. Using the topoisomerase enzyme as opposed to DNA ligase is meant to be quicker and more efficient. I use TOPO TA pCR2.1 vector, which uses the blue white screening system - so you do need x-gal. It works really … incc materiaisSpletDescription It uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants. 3'-T overhangs for direct ligation of Taq -amplified PCR products Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. incc meses 2022Splet10. jul. 2013 · The plasmid backbone is from pCR2.1-Topo (Invitrogen) and contains an ampicillin resistance gene, a kanamycin resistance gene, a pUC ori, a M13 reverse primer and a T7 promoter sequencing primer sites. Restriction enzyme sites are indicated. incc m rsSpletDescription Uses a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants. 3'-T overhangs for direct ligation of Taq -amplified PCR products Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. incc mes outubroSpletStructured Review. Thermo Fisher pcr2 1 topo ta cloning vector. Average 86 stars, based on 1 article reviews. Price from $9.99 to $1999.99. pcr2 1 topo ta cloning vector - by Bioz Stars , 2024-03. 86 / 100 stars. in-box icloud removal downloadSpletInvitrogen TOPO PCR cloning technology was developed to help improve cloning efficiency, simplify protocol setup, and accommodate a wide range of PCR insert sizes. TOPO … incc twitsSpletpCR2.1-TOPO. Parental bacterial expression vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Try SnapGene for Free. … in-box v4.8.0 crack