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Phm6 vector

http://www.protocol-online.org/biology-forums/posts/5295.html WebbpHM6 87228 ™ Organism Yersinia enterocolitica subsp. enterocolitica (Schleifstein and Coleman) Frederiksen Clone type Clone Applications Molecular biology Product format …

Cloning of M and NP gene of H5N1 avian influenza virus and …

Webb21 aug. 2008 · Identification of TUSC4/NPRL2 as a PDK1-interacting protein. (a) After cloning the full-length cDNA of each PDK1 binding protein (BP), we transfected a pc5FLAG vector encoding none (mock, lanes 1 and 2), full-length candidate proteins (BP1-BP8 and TUSC4, lanes 3–11), or 14-3-3θ (lane 12), together with a pHM6 vector encoding none (–, … WebbOpen reading frame of c-myc was cloned into pHM6 vector. These PKM2, PKM2 mutants, and PKM1 expression vector carry mutations at the PKM2 siRNA targeting site, therefore there are resistant to siRNA knockdown. For bicistronic vectors, β-actin ORF was synthesized and inserted into pEGFP-N1 vector with HA tag in N-terminal of . led throne https://ticoniq.com

Ryohei Katayama, Toshiki Watanabe,3 Takashi Tsuruo 2 and …

WebbWe identified p90 ribosomal protein S6 kinases (RSKs; also known as MAPK-activated protein kinases, MAPKAP-K1/K2) as the kinase necessary for Thr 198 phosphorylation. RSK-dependent phosphorylation induced 14-3-3 binding and cytoplasmic localization of p27 Kip1 through a Thr 198 phosphorylation-dependent mechanism. Webb15 apr. 2010 · The PCR amplified merB::flag fragment (0.8 kb) was subcloned into the binary expression vector pHM6, downstream of the E12 promoter, the Cauliflower mosaic virus 35 S promoter with two enhancers in tandem, and an omega sequence, to create the plasmid pBMK01. WebbPuseamp Vector, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 80/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > Upstate Biotechnology Inc > puseamp vector. puseamp vector (Upstate Biotechnology Inc) About; led thread bulb

Ryohei Katayama, Toshiki Watanabe,3 Takashi Tsuruo 2 and …

Category:Addgene: Vector Database - pHM6

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Phm6 vector

(PDF) Freud-1/Aki1, a Novel PDK1-Interacting Protein

WebbBasic Vector Information; Vector Name: pHM6: Antibiotic Resistance: Ampicillin Length: 5450 bp: Type: Mammalian Expression Vectors Selection Marker: … Webb15 juni 2003 · Oligonucleotides carrying Bsu15I and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift from C. H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden) as a template in Sac II- Bsu 15I sites of modified pHM6 …

Phm6 vector

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Webb17 juni 2005 · Human wild-type ILK cDNA (WT-ILK) in a pFLAG-CMV-2 vector and a pHM6 vector were established in our laboratory. Several ILK fragments that encompassed residues 1–406 (ΔC407- ILK ) and 1–375 (ΔC376- ILK ) were generated by converting appropriate codons in WT- ILK cDNA to stop codons using a QuickChange site-directed … http://www.protocol-online.org/biology-forums/posts/5295.html

Webb16 sep. 2009 · The expression plasmid containing the M gene (pHM6-m) or the NP gene (pHM6-np) was then constructed by inserting the M or NP gene into the pHM6 eukaryote … WebbThe wild-type (WT), active (E40K), and dominant-negative (K179A/T308A/S473A, referred to below as AAA) human akt1 cDNAs in the pFLAG-CMV-2 vector (Sigma) or a pHM6 vector (Roche Molecular Biochemicals, Mannheim, Germany) …

WebbVector Type Resistance Marker Bacterial Resistance Source Sequence Available; pHM6: Mammalian Expression: Neomycin: Ampicillin: Roche WebbImmunoprecipitation assay of lysates from Neuro-2a cells transfected with pHM6 vector containing HA-tagged p190RhoGEF-C protein or from Neuro-2a cell transfected with …

Webb16 juni 2014 · OK cells were transiently transfected with pHM6-DNPEP, pHM6-(H94F) DNPEP, or pHM6 empty vector (control) using Lipofectamine 2000 (Invitrogen). Forty-eight hours posttransfection, confluent OK cell monolayers were washed three times in ice-cold PBS, and then biotinylated with EZ-Link NHS-SS- Biotin (Pierce) at 4°C with gentle agitation.

WebbThe S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click … led throughWebb1 aug. 2008 · The HA-tagged protein was obtained from a pHM6 vector encoding no protein (Mock). HA-tagged Identification of the domains responsible for interactions between Freud-1/Aki1 and PDK1. (A) The... led through clear siliconeWebbpHM6 (Search Vector Database) Backbone manufacturer Roche Backbone size w/o insert (bp) 5450 Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance … led through clored lensWebbMammalian Expression Vectors— pHM6 was used to express molecules tagged at the N terminus with hemagglutinin (HA) in mammalian cells (driven by a cytomegalovirus immediate early promoter). Construction of pHM6-Cezanne 1–858 and pHM6-Cezanne 1–443 has been described previously ( 11 ). led through the gardenWebb(a) Immunoblot analysis of MCF7 cells, which were stably transfected with HA-Aurora-A and pHM6 vector, with anti-HA and anti-ERα and anti-actin antibodies. (b–d) Aurora-A- and pHM6... led three way dimmer switchWebbcloned into blunted HindIII(5=)-BamHI(3=) sites of expression vector pHM6 (Invitrogen, Carlsbad, CA), while the 988 bp rat mas PCR fragment was cloned into HindIII (5=)-EcoRI (3=) sites of pHM6 after digestion with HindIII and EcoRI. Chemicals. Mas agonist (AR234960) and inverse agonists (AR244555 and AR305352) were synthesized at Arena … led through holehow to erase comments in pdf