Sagecreation minichemi
WebNov 3, 2024 · Purified AAV-Luc was used to infect the cells at desired m.o.i. for 24 h. The luciferase activities were measured using a luciferase assay system according to the manufacturer’s protocol (Promega, Madison, WI, USA). A luminometer of MiNiCHEMI (Sagecreation, Beijing, China) was used for the quantitative detection of the luminescence. Web凝胶成像_凝胶成像分析系统_化学发光成像仪 -北京赛智创业科技有限公司. 中国生理学会干细胞生理专业委员会第一届学术会议. 相聚南昌-中国植物生理与植物分子生物学学会第十二 …
Sagecreation minichemi
Did you know?
Webby Minichemi® Sagecreation (Baltimore Ave USA). Image J software (National Institutes of Health, Bethesda, MD, USA) was used to analyze and quantify signal intensities. The … http://bio-river.com/wp/?page_id=291
WebMay 17, 2024 · After washing each membrane with TBST (Applygen, Beijing, China) several times, Immobilon Western Chemiluminescent HRP substrate (Millipore, MA, USA) was added, and the blots were analyzed using a MiniChemi chemiluminescence 610 imaging system (Sage Creation, Beijing, China). Protein expression was normalized to β-actin levels.
WebDec 15, 2024 · All antibodies came from Cell Signaling Technology. Detection was performed with ECL substrate (Thermo Scientific, cat. # 32109). Digital images were taken by a MiniChemi from SAGECREATION, Beijing, China. Statistical analysis. Statistical analysis was conducted by using GraphPad Prism (Version 6.0). Data represent the mean ± SEM. WebMar 16, 2024 · Cytochrome P450 26A1 (CYP26A1) plays a vital role in early pregnancy in mice. Our previous studies have found that CYP26A1 affects embryo implantation by modulating natural killer (NK) cells, and that there is a novel population of CYP26A1 + NK cells in the uteri of pregnant mice. The aim of this study was to investigate the effects of …
WebAfter three washes with TBST (1× TBS, 0.02% Tween), the membranes were incubated with secondary antibodies for 1 h at room temperature. After three washes with TBST, signals on the membrane were visualized by ECL (Share-Bio) and scanned by MiniChemi TM (Sagecreation), and the blots were analyzed by Sage Capture Software. Viral Infection
WebMar 2, 2024 · After washing the membranes with PBS, the secondary antibody (goat anti-rabbit IgG(H + L) HRP, 1:1000, Affinity Biosciences) was added and incubated at 37 °C. … immigration medical exam las vegasWebCat.No.29050) on MiniChemi™ (SageCreation) instrument. Quantification of band intensities was processing using Lane 1D 5.0. Cell viability assay In cell viability experiments, cells were suspended in 50 μL of culture medium in 96-well cell culture plate (5000 cells/well). The stock compound in DMSO was diluted with 50 μL of culture medium, immigration medical exam winnipegWebSAGECREATION Service for Life science MÑÌCHEMI MtÑICHeM1 . Title: SAGE2014_1 Author: thco Created Date: 11/27/2014 10:27:15 AM immigration medical exam oakland caWebJan 8, 2024 · Background. Benign prostate hyperplasia (BPH) is one of the most prevalent diseases in aging males with lower urinary tract symptoms. BPH was identified as a disease after the nineteenth century and has distinctive proliferation features through the prostate transitional zone of stromal and epithelial cells. list of the richest people in the world 2023WebAug 15, 2024 · After being incubated with primary antibody and HRP-labeled secondary antibody, the film was developed by using a chemiluminescence detector (Minichemi ™, … immigration medical exam requirementsWebthe MiniChemi™ System (SageCreation, Beijing, China). 2.6ELISA Frozen small intestinal tissue samples (n=6/group) were thawed, homogenized over ice in PBS, centrifuged and … immigration medicals taurangaWebMar 1, 2024 · After being sealed with 5% skim milk powder for 1 h, the antibody of the target protein was added and incubated at 4 °C overnight. Finally, after cleaning the PVDF membrane, the second antibody was incubated for 1 h. A Chemiluminescence imaging analysis system was used for protein detection (MiniChemi 610 plus, Sagecreation, China). immigration medical whangarei